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View full-text article in PMC

. 2013 Oct 8;8(10):e76947. doi: 10.1371/journal.pone.0076947

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© 2013 Leyton et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunoblots of lysates of WT (Bmpr2^+/+) or Bmpr2 ^Δtd/+ PaSMCs treated with BMP4 or BMP7 (10 ng/ml) for various times were reacted with antibodies directed against phosphorylated and total Smad1/5/8. Quantification of the ratio of phosphorylated Smad1/5/8 to total Smad1/5/8 (analysis of 3 independent experiments) demonstrated that BMP4 signaling is similar in Bmpr2 ^+/+ or Bmpr2 ^Δtd/+ PaSMCs, whereas BMP7 signaling is greater in Bmpr2 ^Δtd/+ PaSMCs. *P<0.05 compared to Bmpr2 ^+/+ PaSMC group treated with BMP7. Id1 (B) and Smad6 (C) mRNA levels were measured by qPCR in Bmpr2 ^+/+ or Bmpr2 ^Δtd/+ PaSMCs treated with BMP4 or BMP7 (10 ng/ml) for various times. Id1 and Smad6 gene expression was normalized to Gapdh and expressed as fold-change relative to control Bmpr2 ^+/+ PaSMC group. *P < 0.01 compared to Bmpr2 ^+/+ PaSMC group treated with BMP7.