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. 2013 Oct 8;8(10):e76947. doi: 10.1371/journal.pone.0076947

Figure 4. BMP7 signaling in Bmpr2 Δtd/+ and Bmpr2 Δtd/del PaSMCs does not depend on the presence of Acvr2a.

Figure 4

(A) Bmpr2 Δtd/+ PaSMCs were treated with a siRNA specific for Acvr2a transcripts. The ability of BMP4 or BMP7 (10 ng/ml for 1.5 h) to induce Id1 and Smad6 gene expression was measured by qPCR, normalized to Gapdh and expressed as fold-change relative to Bmpr2 Δtd/+ PaSMCs treated with siNC. *P < 0.01 compared to siNC within BMP treatment. Silencing efficiency was quantified by measuring Acvr2a mRNA levels. (B) Bmpr2 Δtd/del PaSMCs were treated with siAcvr2a. The ability of BMP4 or BMP7 (10 ng/ml for 1.5 h) to induce Id1 and Smad6 gene expression was measured by qPCR, normalized to Gapdh and expressed as fold-change relative to Bmpr2 Δtd/del PaSMCs treated with siNC. *P < 0.01 compared to siNC within BMP treatment. Acvr2a silencing efficiency was measured by qPCR.