Table 2. Priming details for 20 novel nuclear markers.
|
Primers (Forward, Reverse)
|
||||
|---|---|---|---|---|
| Protein Region | Name | Sequence (5'–3') | PCR Program | |
| ApPEFP_C | 1 | 4218Cf4, 4218Cr12 | GGACCTGGSCTYGCTGARGAGTG, GCAACRTGAGCAGCYGGTTCRCGRGG | 6512035 |
| ApPEFP_C | 1a | 4218Cf4, 4218Cr3 | GGACCTGGSCTYGCTGARGAGTG, TCGTAAGCRTTYGTTACTTTDGCC | 5506035 |
| ApPEFP_C | 1b | 4218Cf6, 4218Cr6 | AAAGTTATACATACTGTTGGTCC, GCAACATGAGCAGCTGGTTCACGAGG | 5506035 |
| ApPEFP_C | 2 | 4218f25, 4218r7 | AATGCTCTRAGTCAYTGYTAYMGATC, TTGTAAATCTCTGTRTCRGATGYYGT | 5509035 |
| ApPEFP_C | 3 | 4218f26, 4218r13 | CAAAGGCGCAARGAACARTGGGARAGRGTTGC, TCAAGACAYCGTAGCAGRAARTGBGCYCC | 6512035 |
| CRY2 | 1 | CRY2F3289_Pt, CRY2R3838_Pt | AGGATGARYTGGAGAAAGGYAGCAATG, GTRTCCCAGAAATAYTTCATACCCC | 5209035 |
| CRY4 | 1 | CRY2F3289_Pt, CRY2R3838_Pt | AGGATGARYTGGAGAAAGGYAGCAATG, GTRTCCCAGAAATAYTTCATACCCC | 5209035 |
| DET1 | 1 | det1-335all, det1-906all | TATGAYGTGGARTGCCCAGAT, TCTCTGCAGAAHKGYCCAA | 5506035 |
| gapCpSh | 1 | gapCpShF1, gapCpShR2 | TGCACMACHAACTGCCTTGCRCCBCTTGC, CCATTYARCTCTGGRAGCACCTTTCC | 6512035 |
| IBR3 | 1 | 4321F2, 4321R2 | TCTGCMCATGCMATTGAAAGAGAG, CCCARKGTYGAAAGYTCCCAATC | 6312035 |
| IBR3 | 2 | 4321F5, 4321R6 | ATGACYGAACCAGATGTKGCDTCVTCRGATGC, TGRTGGAGYCTKCCTGGGCCTA | 6512035 |
| pgiC | 1 | pgic_1156F, pgiC_1900R | GGYCTYYTRAGYGTYTGGAATGT, GGTGAAATYGAYTTYGGDGARC | 5812035 |
| SQD1 | 1 | EMSQD1E1F6, EMSQD1E1R2 | GCAAGGGTACHAAGGTHATGATCATAGG, CCTTTDCCRTARACTGTAAGAGGATG | 5512035 |
| SQD1 | 1a | EMSQD1E1F6, EMSQD1E1R4 | GCAAGGGTACHAAGGTHATGATCATAGG, GCGTGARTCRTGCACTTTGCTRAGATG | 5512035 |
| SQD1 | 2 | EMSQD1E2F4, EMSQD1E2R8 | CGHGTRTTYAATCARTTYACAGAAC, GTCACTGTHACAGGTTTYACDCCAGC | 5512035 |
| TPLATE | 1 | 6560_1630F, 6560_2329R | TGCYTAGTSGARAGYTGYTTTCA, AATGTAGCAACTAACAGGCTTCAAGA | 5812035 |
| TPLATE | 2 | 6560_3136F, 6560_3686R | AAYCTYCARCATCTYCAGTCTC, GCAACKGCHGCDGTBGAAAG | 5812035 |
| transducin | 1 | 6928_850F, 6928_1357R | TTRCGBGGRCAYARAGATCA, GGAWCSTTARTSGGYTGCCAA | 5812035 |
| transducin | 2 | 6928_1955F, 6928_2816R | AAGGCDGGRAARCTNGAGAT, ATGGAYATYTCCWCYGATGC | 5812035 |
| transducin | 3 | 6928_3406F, 6928_3802R | TCBATTCGRMGATGGGAGCG, CAAACYCARGARWCYSTGAC | 5812035 |
The first two digits of the PCR program is the annealing temperature, followed by a three-digit elongation time (in seconds), followed by the number of cycles.