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. 2013 Oct 8;8(10):e76792. doi: 10.1371/journal.pone.0076792

Figure 4. Localized interaction between Htt and Grb2 and Chaperone potential of Grb2.

Figure 4

A. Normalized fold change of luciferase signal of cells transfected with pGL3 basic vector, pGL3 vector plus Dsred empty vector, pGL3 basic vector plus Grb2-Dsred and pGL3 vector plus Hsp70 GFP, all of the cells given heat shock for 1 hour and recovery at 37°C for 0hr, 2hr and 6hr. Fold change was calculated taking no heat shock cells as a control. B. Change in relative absorbance at 360nm with time was plotted for insulin with DTT alone or, in presence of 0.3mg/ml BSA, 0.3mg/ml Grb2, and 0.3mg/ml Hsp70. C. Fluorescence lifetime images of 145Q Httex1 GFP in cells transfected with i. 145Q Httex1 GFP, ii. co-transfected with 145Q Httex1 GFP and Grb2-Dsred and iii.co-transfected with 145Q Httex1 GFP and DsredC1. D. Fluorescence lifetime images of 23Q Httex1 GFP in cells transfected with i. 23Q Httex1 GFP, ii. Co-transfected with 23Q Httex1 GFP and Grb2-Dsred C and iii. co-transfected with 23Q Httex1 GFP and DsredC1. E. Analysis of cells co-transfected with 145Q Httex1 GFP and Grb2-Dsred i. a1/a2 image shows the ratio of interacting and non interacting species within the cell, ii and iii (normalized and not normalized images respectively) shows τ1/τ2 images in different scales showing the ratio of GFP two lifetime species within the cell.