Figure 11.

Effect of nocodazole on VacA-induced redistribution of late endocytic compartments. HeLa cells were transiently transfected with pEGFP-Rab7. (A and B) After pretreatment with 2 μg/ml nocodazole for 1 h, cells were either treated with acid-activated VacA (5 μg/ml) (B) or left untreated (A), and incubated at 37°C for 16 h. (C) Transfected HeLa cells were incubated with VacA for 16 h in the absence of nocodazole. (D) Nocodazole-pretreated cells were incubated with VacA for 16 h and then incubated with 5 mM ammonium chloride for 1 h. Nocodazole did not inhibit VacA-induced clustering of Rab7-containing compartments or development of vacuoles from clustered compartments upon ammonium chloride supplementation, but it did prevent redistribution of clustered compartments to the perinuclear region. Bar, 10 μm.