Figure 7.

Cells expressing dominant-negative Rab7 are resistant to VacA-induced vacuolation. HeLa cells were transiently transfected with pEGFP-Rab7Q67L (constitutively active mutant) (A-C) or pEGFP-Rab7T22N (dominant-negative mutant) (D-F). Twenty-four hours after transfection, HeLa cells were treated with 5 μg/ml acid-activated VacA in the presence of 5 mM ammonium chloride for 8 h. Cells expressing the dominant-negative Rab7 protein (Rab7T22N) did not become vacuolated in response to VacA (F). Bar, 10 μm.