Figure 8.

Effect of VacA on distribution of constitutively active or inactive Rab7. HeLa cells were transiently transfected with pEGFP-Rab7Q67L (encoding constitutively active mutant Rab7) (A and B) or pEGFP-Rab7T22N (encoding dominant-negative mutant Rab7) (C and D). Twenty-four hours after transfection, HeLa cells were treated with 5 μg/ml acid-activated VacA for 20 h without supplemental ammonium chloride (B and D) or left untreated (A and C). VacA induced redistribution of the active mutant EGFP-Rab7Q67L (B), whereas the dominant-negative mutant EGFP-Rab7T22N maintained its diffuse distribution in the cytosol (D). Bar, 10 μm.