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. 2013 Oct 8;8(10):e76721. doi: 10.1371/journal.pone.0076721

Figure 4. Expression of mitochondrial proteins in NPCs during differentiation.

Figure 4

(A, B and C) Immunoblot analysis was performed by using antibodies that selectively recognize cytochrome c, MnSOD and cyclophylin D. Blots were reprobed with antibodies against Actin. Panel A shows representative blots; panels B and C show results of densitometric analysis of cyclophylin D and MnSOD, which were normalized to the corresponding mitochondrial cytochrome c level on each differentiation day. Values are expressed as a percentage of the mean of NPCs at day 0 (n = 3-4 separate experiments performed on cells cultured from 3-4 pregnant mice); *p<0.05 compared to the values of NPCs at day 0.