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. 2013 Oct 8;8(10):e77320. doi: 10.1371/journal.pone.0077320

Figure 2. HIV-1 Vpr decreases the expression of sZIP in a dose-dependent manner.

Figure 2

A. HeLa cells were co-transfected with a vector expressing FLAG-sZIP and with increasing amounts of a vector expressing HA-tagged Vpr. A GFP expression vector was used as an internal transfection control. Cells were harvested 48h post-transfection, lysed and protein expression analyzed by Western Blot (top panel). The histogram (bottom panel) displays the ratio between the FLAG signal and the GFP signal compared to this ratio without Vpr. B. Same as in A except with increasing amounts of the vector expressing FLAG-sZIP, with or without HA-tagged Vpr.