Figure 1. Mitochondrial DNA copy number analysis and mitochondrial transmembrane potential.

A) Single cell mtDNA copy number distributions in metaplastic (CP-A) and dysplastic (CP-C) cells, B) box charts of the two distributions shown in panel A. Following values are depicted: open square – mean; solid line – median; upper and lower box lines – the 75^th and 25^th percentiles, respectively; upper and lower whiskers – the 95^th and 5^th percentiles, respectively; x – minimal and maximal values of the distribution. The p value of <0.002 was calculated using the two-tailed non-parametric Mann-Whitney statistical significance test (n = 99); mtDNA copy numbers range between 150–1,900 C–D) JC-1 fluorescence micrographs of hypoxic CP-A (C) and hypoxic CP-C (D) cells; single cell distributions of the relative mitochondrial membrane potential in CP-A (E) and CP-C (F) cells. JC-1 signals from about 100 cells per cell line and condition were analyzed; G) statistical representation of the MMP distribution in both cell types/conditions.