Figure 9. Cell toxicity of ΔIWF mutants.

The percentage of GFP positive cells was determined in co-transfection experiments at 24 (dark gray) or 48 (light gray) hours post-transfection (see Materials and Methods section). Scoring was determined in blind experiments by counting 1000–1500 cells (i.e. DAPI staining) from three randomly selected microscope fields. Data are expressed as mean±SD of two independent experiments. The symbols (**) and (*) indicate significant difference vs. DUX4 wt, p<0.01 and p<0.05, respectively.