Figure 3. The diversity of the Predator library.

The CDR2 and CDR3 of the library have been designed with specific frequencies designated to every amino acid used for all the randomized position (red boxes) in both CDR2 and CDR3 according to the different properties of the amino acids. The frequency of a given amino acid at a given position can be read from the table. The unique restriction sites used for cloning the CDR regions into the vector are shown flanking the sequences.