Figure 4. Sequences showing an error prone repeat close to primer annealing site.

The sequencing data from eight clones with errors and a reference sequence showing the CDR2 and immediate downstream region. The nucleotides composing the randomized region of CDR2 are indicated at the top along with the nucleotides matching the 3’ end of the reverse primer used for the generation of the CDR2 insert. Eight of the shown clones have errors and all of the errors have occurred in the area between the randomized region of CDR2 and 3’ end of the reverse primer used in the generation of the CDR2 insert. Especially the “TATTAT” repeat seems susceptible to errors.