Figure 2. Immunophenotyping of reticulocyte samples.

Difference of between mean fluorescence intensity with different erythrocytic antigens and mean fluorescence intensity with secondary antibody on reticulocytes sampled from the four CD71 gates (CD71^high, CD71^medium, CD71^low and CD71^negative) (Delta of Mean Fluorescence Intensity (MFI)). The dotted red line represents the threshold of positivity based on the CD15 staining.