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. 2013 Oct 8;8(10):e75751. doi: 10.1371/journal.pone.0075751

Figure 1. Dispensable role of hXRCC3 in the delay of S-phase induced by UVAMEMi and in protecting cells from UVA phototoxicity.

Figure 1

(A) CHO DRA10 cells were exposed to increasing doses of UVA in MEMi or PBS and the number of G418R clones scored after selection. Results are the mean ± SD of three independent experiments. (B) XRCC3 proficient (CXR3) and deficient (irs1SF) CHO cells were labeled with BrdU, irradiated at 120 kJ/m2 UVAMEMi and trypsinized after 6 hours of recovery. Cells were fixed in 70% EtOH and analysed by flow cytometry for BrdU incorporation (FITC-BrdU) and DNA content (PI). The data shown are representative of two independent experiments. (C) XRCC3 proficient and deficient cells were exposed to increasing doses of UVAMEMi, trypsinized, and plated at low density. The colonies were stained after 8–10 days incubation at 37°C. Results are the mean ± SD of three independent experiments.