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. 2013 Oct 8;8(10):e77112. doi: 10.1371/journal.pone.0077112

Figure 4. Detection of surface-immobilized anti-CD34 antibodies using quantum dots.

Figure 4

When films were immunostained with red QD-IgG fluorescent labels, (A) control POSS-PCU and (B) POSS-PCU-FS did not exhibit fluorescence, as compared to (C-D) POSS-PCU-FS+CD34 films, which showed uniform immobilization of bound antibodies on the film even after washing by mechanical shaking for 24 and 72 hrs (10x magnification). (E) Measurements of samples’ residual fluorescent intensities after 24 and 72 hrs of washing demonstrates that antibodies remain stably bound. Error bars: ± SD; NS denotes no significant difference (p > 0.05).

POSS-PCU-FS: POSS-PCU with fumed silica anchors, POSS-PCU-FS+CD34: POSS-PCU biofunctionalized with anti-CD34 antibodies.