Figure 9. EPC capture assay.
Cells were double-stained at day 7 for either EPC (CD34/VEGFR-2) or EC (CD31/vWF) markers, and counterstained with DAPI for nuclei. (A) Positive control, (B) POSS-PCU-FS+CD34, (C) POSS-PCU-FS, (D) POSS-PCU, (E) POSS-PCU-FS+IgG isotype negative control (20x magnification). Scale bar represents 20 µm.
POSS-PCU-FS: POSS-PCU with fumed silica anchors, POSS-PCU-FS+CD34: POSS-PCU biofunctionalized with anti-CD34 antibodies, POSS-PCU-FS+IgG: POSS-PCU biofunctionalized with goat anti-mouse antibodies.