Ethylene triple response assay. A, Seedlings of wild-type Ailsa Craig (AC++) and RNAi lines (RNAi-03 and RNAi-16) treated with 0 and 5.0 µm
ACC. B and C, Elongation of hypocotyl (B) and root (C) growth on different concentrations of ACC. Error bars represent ± se. D, Expression of ACS1A, ACS2, ACS6, and ACO1 in seedlings of RNAi lines and the wild type (WT). E, Expression of SlMADS1 in seedlings of the wild type treated with 0 (A0), 1.0 (A1), 2.0 (A2), 5.0 (A5), 10.0 (A10), and 20.0 (A20) µm
ACC. [See online article for color version of this figure.]