Figure 4.

Changes in the degree of oxidation of roGFP in various cellular compartments during developmental senescence. The degree of roGFP oxidation in transgenic Arabidopsis lines expressing roGFP2 in plastids, mitochondria cytoplasm, and peroxisomes was determined following measurements of fluorescence at 515 nm resulting from excitation at 400 or 485 nm using a fluorometer as described in “Materials and Methods.”