Figure 5.

Induction of stomatal closure and inhibition of light-induced stomata opening by ABA in the wild type (WT) and the srk2e mutant. A, ABA-induced stomatal closure in wild-type and srk2e plants. Averages from eight independent experiments (n = 8; 160 stomata per bar) are shown. B, ABA inhibition of stomatal opening in wild-type and srk2e plants. Dark represents fully dark-adapted stomata; 0 µm ABA indicates 2.5-h light treatment after the dark adaption with 0.1% ethanol as the solvent control; 1 and 10 µm ABA represent an ABA addition at same time as the initiation of light treatment. Averages from three independent experiments are shown (n = 3; 60 stomata per bar). Asterisks indicate significant differences (**α = 0.01) by Student’s t test. Error bars represent se.