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. 2013 Aug 6;163(2):746–756. doi: 10.1104/pp.113.223412

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Figure 5. — Transient GUS reporter gene expression in transformed tobacco leaf. Tobacco leaves were cotransformed with a combination of regulator and reporter plasmids as indicated. Means and se of 12 independent measurements (three biological replicates with four technical replicates each) of GUS enzyme activities are indicated. An antibody against the HA tag was used to detect HA-tagged WHY1 proteins. oeWHY1-HA is overexpressing WHY1-HA; Pwrky53:GUS is GUS driven by the WRKY53 promoter; Pwrky53m0:GUS is GUS driven by the m0-mutated WRKY53 promoter; Pwrky53m12:GUS is GUS driven by the m12-mutated WRKY53 promoter; 4MUG is 4-Methylumbelliferyl-β-D-glucuronide.