Figure 4.

Functional assay of HvPhyC in rice by introducing different HvPhyC alleles (T1 generation). Mutant allele (HvPhyC-e; red-filled marks) and wild-type allele (HvPhyC-l; blue-filled marks) were introduced into rice phyA phyC double mutant lines with a var. Nipponbare genetic background via the A. tumefaciens-mediated transformation method. As the control, empty vector was introduced into phyA phyC double mutant (phyA phyC mock; open red circle) and var. Nipponbare (Nip mock; open blue triangle). All of the T1 lines were grown under natural (long) photoperiod conditions in a greenhouse. Expression level of HvPhyC was analyzed by semiquantitative RT-PCR using OsActin as the internal control.