Figure 3.

PLDδ is the only PLD isoform contributing to penetration resistance against Bgh. A, Bgh penetration rate of pldδ leaves infiltrated with water and 0.6% n-butanol or tert-butanol. No significant difference in penetration was observed for pldδ + n-butanol, indicating that no other PLD isoform contributes to penetration resistance. B and C, Bgh penetration rate at 2 dpi on leaves of different 3-week-old PLD mutant plants. KO mutations of PLDα1 (B) or PLDβ1 and PLDβ2 (C) in the pldδ background do not cause a further increase in Bgh penetration rate. D, PLDδ KO in the pen1 or pen3 mutant background does not result in any further increase of Bgh penetration frequencies. Lowercase letters indicate significant differences between genotypes (P < 0.05) as determined by multiple comparison using one-way ANOVA, with 3 (A–C) or 5 (D) degrees of freedom. Means ± sd are shown (n = 4). Three independent experiments were performed with similar results.