Figure 1.

SFG spectra of ground wall pellets from different axial locations of stems from 6WAI (A) and 8WAI (B) of wild-type (Col-0) plants. Inflorescence stem regions are as follows: B, bottom (5%–20%); BM, bottom-middle (20%–40%); M, middle (40%–60%); MT, middle-top (60%–80%); T, top (80%–95%). C, Correlations of SFG intensity of the 2,944 cm⁻¹ peak with crystalline cellulose values of 6WAI and 8WAI (r² = 0.91; n = 5 for SFG, n = 3 for 6WAI and 8WAI crystalline content). D, OH (3,320 cm⁻¹)/CH₂ (2,944 cm⁻¹) SFG intensity ratio of 6WAI and 8WAI of the wild type (Col-0). Error bars represent se (8 ≤ n ≤ 10). Some error bars are not seen because they are too small. SFG experiments (A and B) were repeated three times with similar results. a.u., Arbitrary unit. [See online article for color version of this figure.]