Figure 2.

SFG measurements of the inside and outside from 8-week-old wild-type (WT) Landsberg erecta (Ler), irx1-1, and irx3-1 inflorescences. A, Diagram of an intact Arabidopsis inflorescence sample for SFG analysis. Inside (stele) and outside (epidermis) tissues were exposed to IR and Raman spectroscopy for SFG measurement as illustrated. B, The inflorescence stems were cut in the middle region (see “Materials and Methods”). SFG measurements were repeated three times with similar results. a.u., Arbitrary unit. [See online article for color version of this figure.]