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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Mol Microbiol. 2013 Aug 19;90(1):54–71. doi: 10.1111/mmi.12347

Figure 3. DivJ is a kinase that phosphorylates DivK in vitro.

Figure 3

A. Purified DivJ histidine kinase domain is able to auto-phosphorylate using ATP as the phosphate source. DivJ in presence of ATP gives two distinct bands in a SDS-PAGE Phos-tag™ gel (Coomassie blue). In particular the amount of phosphorylated band (upper band) increases over time; B. The mutant D53A is not able to receive the phosphate from DivJ.