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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Mol Microbiol. 2013 Aug 19;90(1):54–71. doi: 10.1111/mmi.12347

Figure 5. DivJ and CbrA are inhibiting CtrA phosphorylation and activity in vivo.

Figure 5

A. β-galactosidase activity of a CtrA-controlled promoter in wild type (EB594), ΔdivJ (EB638) and cbrA∷ Tn5 (EB593) genetic backgrounds. Experiments were performed in biological triplicates, errors are calculated as standard deviations. Asterisk corresponds to significant statistical difference with wild type conditions (Student’s test); B. Phos-tag™ SDS-PAGE gel shows phosphorylation of CtrA in vivo in wild type. A lane with SDS-lysed material (Phos.) and another with boiled sample are shown. The phosphorylated band disappears after boiling; C. Quantification of phosphorylation levels of CtrA in vivo in wild type, ΔdivJ and cbrA∷ Tn5 genetic backgrounds. The average of three experiments using samples at the same OD600 is showed, errors are calculated as standard deviations. The amount of CtrA-P was normalized for the number of cells. Asterisk corresponds to significant statistical difference with wild type conditions (Student’s test).