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Original recording of the lumen-positive transepithelial voltage (Vte) and voltage deflections (Δ V0) in an isolated, perfused mouse medullary thick ascending limb (mTAL). Steady state conditions were reached after about 15 min. The arrow indicates the time point used to quantify transport. In addition, the reversible blocking effect of luminal furosemide (100 μ M) is shown.
