Figure 3.

Bim interactions in control and seizure brain. (A) Bim was immunoprecipitated (IP) from control or seizure hippocampus 4 hours after diazepam administration (n = 2 per lane), and then lysates were immunoblotted (IB) to detect the presence of interacting proteins. Bim binds to dynein, Bcl-w, and 14-3-3 in control brain, but not Bax, Bcl-2, or Bcl-xl. Following seizures, Bim interactions with dynein and 14-3-3, but not Bcl-w, declined. IgG levels confirm equivalency of antibody loading. Whole cell lysates run concurrently are shown to the left to confirm molecular weight (+). (B) Representative Western blots (n = 2 per lane) showing expression of each interacting protein in hippocampal lysates. Immunoblots are representative of two or three independent experiments.