Table 2.
Primers used to screen for antimicrobial resistance genes in M. haemolytica isolated from feedlot cattle.
| Resistance phenotypea | Resistance gene | Primer sequences 5′–3′ | Annealing (°C) | Reference |
|---|---|---|---|---|
| Amp, Pen | blaROB-1 | AATAACCCTTGCCCCAATTC | 60 | Klima et al. (2011) |
| TCGCTTATCAGGTGTGCTTG | ||||
| Neo | aphA-1 | TTATGCCTCTTCCGACCATC | 54 | Klima et al. (2013) |
| GAGAAAACTCACCGAGGCAG | ||||
| Tet | tet(H) | ATACTGCTGATCACCGT | 60 | Klima et al. (2011) |
| TCCCAATAAGCGACGCT | ||||
| Til, Tul | erm(A) | GAAATYGGRTCAGGAAAAGG | 55 | Chen et al. (2007) |
| AAYAGYAAACCYAAAGCTC | ||||
| erm(B) | GATACCGTTTACGAAATTGG | 58 | Chen et al. (2007) | |
| GAATCGAGACTTGAGTGTGC | ||||
| erm(F) | CGACACAGCTTTGGTTGAAC | 56 | Chen et al. (2007) | |
| GGACCTACCTCATAGACAAG | ||||
| erm(X) | GAGATCGGRCCAGGAAGC | 58 | Chen et al. (2007) | |
| GTGTGCACCATCGCCTGA | ||||
| erm(42) | GGGTGAAAAGGGCGTTTATT | 60 | Kadlec et al. (2011) | |
| ACGTTGCACTTGGTTTGACA | ||||
| msr(E)-mph(E) | TACCGGAACAACGTGATTGA | 60 | Kadlec et al. (2011) | |
| GAAGGGTTACGCCAGTACCA |
a
Amp, ampicillin; Neo, neomycin; Pen, penicillin; Tet, tetracycline; Til, tilmicosin; Tul, tulathromycin.