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. 2004 Apr 1;113(7):1025–1031. doi: 10.1172/JCI20106

Figure 2.

Figure 2

Engineered hFVIIa transgenes and in vitro characterization. (A) Schematic representation of the engineered hFVII and hFVIIa constructs, showing the light and heavy chains. An open arrow indicates the proposed recognition site of the PACE/furin type intracellular protease. (B) PAGE gel under reducing conditions of 4 μg of in vitro–expressed and –purified engineered transgenes: far left lane (labeled S, FVIIa) contains rhFVIIa as a reference standard; lane 1, hFVII-WT; lane 2, hFVII-RKR; lane 3, hFVII-2RKR; lane 4, hFVII-INS. Arrows point to the following forms: zymogen (Z), heavy chain, and light chain. Molecular weight markers are shown to the left in kilodaltons. (C) In vitro activity based on PT as indicated on top of each bar (mean %), relative to rhFVIIa (100%): far left, hFVII-WT; center left, hFVII-RKR; center right, hFVII-2RKR; far right, hFVII-INS. Error bars denote mean ± 1 SD.