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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Anal Biochem. 2008 Nov 27;386(2):194–216. doi: 10.1016/j.ab.2008.11.021

Fig. 3.

Fig. 3

Types of assay format used in the study. (A) Classic: several analyte concentrations flowed serially across an immobilized ligand with a surface regeneration step between each injection. (B) SNL (short-‘n-long) dissociation: several analyte concentrations with a relatively short dissociation phase and one analyte concentration with a much longer dissociation phase flowed serially across an immobilized ligand with a regeneration step between each injection. (C) One-shot: several analyte concentrations flowed in parallel across a ligand surface without surface regeneration. (D) Kinetic titration: several analyte concentrations flowed serially over a ligand surface without surface regeneration. (E) Ligand array: a single analyte concentration flowed simultaneously across multiple immobilized ligand spots of different densities without surface regeneration. In this nine-spot array example, the ligand was spotted three times at three concentrations. RU, resonance units.