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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Anal Biochem. 2008 Nov 27;386(2):194–216. doi: 10.1016/j.ab.2008.11.021

Fig. 4.

Fig. 4

Preimmobilization tests of Fab (left) and Ag (right). (A) Nonspecific binding test: the responses from injections of a buffer blank and 100 nM protein across an unmodified sensor chip are overlaid. (B) pH scouting: 100 nM protein was injected at different pH levels to identify the optimal pH for ligand preconcentration. RU, resonance units.