Figure 1.

Low frequencies of peripheral virus-specific CD8⁺ T cells expressing a dominant CM (CCR7⁺) phenotype. PBMCs from two representative HLA-A2⁺ patients (Pt. 1 and Pt. 15) and two HLA-A3⁺ patients (Pt. 9 and Pt. 11) were stained first with rat mAb to CCR7 and then with secondary FITC-conjugated goat anti-rat Ig, PE-Cy5–labeled anti-CD8, and the indicated PE-Cy5–labeled tetramers. (A) Dot plot analyses showing CD8⁺tetramer⁺ cell percentages. (B) Dot plots, corresponding to those reported in A, are gated on tetramer⁺CD8⁺ (Tetr⁺CD8⁺) cells, and show CCR7 staining. Results are expressed as percentage of cells, indicated in each quadrant. N.D., not determined; FSC, forward scatter.