FIGURE 2 .

Cells were either treated with 2 U/ml thrombin for 4 hours with or without pretreatment with 60 mM PAR-1 siRNA or 1 μM argatroban for 30 minutes. A549 cells were transfected with PAR-1 siRNA using transfection reagent for 6 hours at 37°C, washed using 2x normal growth media containing antibiotics and incubated in 1x normal growth media. Cells were also treated either with 300 μM TFLLR or 10 ng/mL TGF-β for 4 hours for real-time PCR. Thrombin and TFLLR increased α-SMA and collagen I mRNA expression and suppressing E-cadherin mRNA expression by quantitative real-time PCR. PAR-1 siRNA or pretreatment with argatroban inhibited thrombin-induced α-SMA and collagen I mRNA expression, and reversed thrombin-induced suppression of E-cadherin mRNA expression. Data are means ± SE; n = 5/group. *, † P < .05; ** P < .01. *, **; compared with control. ^{†, ††}; compared with thrombin.