FIGURE 3 .

Cells were either treated with 2 U/mL thrombin for 72 hours with or without pretreatment with 60 mM PAR-1 siRNA or 1 μM argatroban for 30 minutes. A549 cells were transfected with PAR-1 siRNA using transfection reagent for 6 hours at 37°C, washed using 2x normal growth media containing antibiotics and incubated in 1x normal growth media. Cells were also treated either with 300 μM TFLLR or 10 ng/mL TGF-β for 72 hours for immunoblotting. Thrombin and TFLLR increased α-SMA and collagen I protein expression while PAR-1 siRNA transfection or pretreatment with argatroban reversed thrombin-induced α-SMA and collagen I expression as assessed by Western blot. PAR-1 siRNA or pretreatment with argatroban reversed thrombin-induced suppression of E-cadherin expression. Data are means ± SE; n = 5/group. *, † P < .05; **, †† P < .01. *, **; compared with control. ^{†, ††}; compared with thrombin.