Figure 2.

Inhibition of Ab-induced platelet fragmentation with classic inhibitors of the NADPH oxidase pathway. Gel-filtered human platelets were preincubated with inhibitors at various concentrations for 15 minutes prior to the addition of 25 μg/ml affinity-purified anti–GPIIIa49–66 (AP) or control (C) IgG for 4 hours. Ci, control IgG plus inhibitor; BPB, bomophenacyl bromide. Platelet fragmentation was monitored by flow cytometry of platelets labeled with anti–CD41-FITC Ab. Platelet fragments were monitored by enumeration of fluorescent particles in gates established to detect smaller particles. A, n = 4; B, n = 4; C, n = 3; D, n = 6.