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. 2004 Apr 1;113(7):973–980. doi: 10.1172/JCI20726

Figure 5.

Figure 5

Effect of 12(S)-HETE on platelet oxidation. (A) Gel-filtered human platelets were preincubated with various concentrations of catalase and DPI for 15 minutes prior to the addition of 12(S)-HETE (100 nM) for 4 hours. Cb, control buffer; H, 12(S)-HETE with no drug. Increasing numbers refer to doubling concentrations of catalase, starting at 125 μg/ml, and DPI, starting at 10 μM. (B) Gel-filtered platelets were loaded with DCFH and incubated with 12(S)-HETE at various concentrations for 4 hours at 37°C. Oxidation was measured by DCFH fluorescence.