Figure 3.

Influenza titers were controlled through extracellular MPO and on transcriptional level through PAR₂. (a, b) DF from stimulated neutrophils was supplemented with H₂O₂, and the virucidal activity was determined. DF from PAR₂ agonist treated neutrophils disrupted IAV. (c) Application of a MPO inhibitor enhanced viral titers. Interestingly, despite MPO inhibition, PAR₂ activation reduced viral replication in neutrophils. (d) Analysis of viral gene replication displayed reduced NS-1 mRNA expression in PAR₂ agonist stimulated neutrophils. IFNγ had no effect on viral replication. For student's t-test: ^#,∗ P < 0.05; **P < 0.01; ***P < 0.005. The symbol ∗ marks the significance as compared to control and the symbol # as compared to IFNγ sample.