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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Dev Cell. 2004 Sep;7(3):347–358. doi: 10.1016/j.devcel.2004.08.014

Table 1.

Cooperative Activation of Chordin and C-TSK

Constructs Number of
Secondary Axis		 Number of
Embryos		 Ratio/% p Value
tBR, 1.25 pg 0 51 0
tBR, 2.5 pg 1 58 1.72
tBR, 5 pg 2 51 3.92
tBR, 10 pg 16 64 25.0
chordin, 1.25 pg 0 81 0
chordin, 2.5 pg 0 128 0
chordin, 5 pg 1 57 1.75
chordin, 10 pg 21 51 41.2
C-TSK, 6.25 pg 0 73 0
C-TSK, 12.5 pg 8 220 3.64
C-TSK, 25 pg 65 200 32.5
C-TSK, 37.5 pg 66 134 49.3
tBR, 1.25 pg + C-TSK, 12.5 pg 7 74 9.45 0.489
tBR, 2.5 pg + C-TSK, 12.5 pg 16 68 23.5 1.07
tBR, 2.5 pg + C-TSK, 25 pg 35 72 48.6 1.25
tBR, 5 pg + C-TSK, 25 pg 16 43 37.2 0.82
chordin, 1.25 pg + C-TSK, 12.5 pg 55 90 61.1 2.83
chordin, 2.5 pg + C-TSK, 12.5 pg 48 93 51.6 1.93
chordin, 2.5 pg + C-TSK, 25 pg 78 88 88.6 2.05
chordin, 5 pg + C-TSK, 12.5 pg 51 90 56.7 1.53

After overexpression of the indicated constructs, their effects on the secondary axis formation were evaluated. The p values were determined by the equation described in Experimental Procedures.