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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Methods Enzymol. 2005;393:288–301. doi: 10.1016/S0076-6879(05)93012-7

TABLE I.

Contents for HBSS (Final Volume of 1 Liter)a

  1. Hanks’ balanced salt solution 10X (H1641, Sigma Chemical Co., St Louis, MO) 100 ml

  2. Sodium bicarbonate solution (S8761, 7.5%, Sigma) 4.7 ml

  3. I M HEPES buffer (#H0887, Sigma) 10 ml

  4. Penicillin–streptomycin (#15140-122, 10,000 unit/ml–10,000 μg/ml, Gibco, Invitrogen Co., Grand Island, NY) 10 ml

a

All contents should be dissolved in autoclaved Milli-Q water; the total volume should be adjusted to 1 liter and kept at 4°.