Skip to main content
. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Biochim Biophys Acta. 2012 Feb 9;1823(4):889–899. doi: 10.1016/j.bbamcr.2012.01.017

Fig. 4.

Fig. 4

Characterization of the hMSH3 promoter region. A: Reporter constructs containing different regions of the hMSH3 5′ sequences and their reporter activities in SW620 or HeLa S3 cells. The activity of pGL3-promoter was defined as 1 and the relative luciferase activity was determined. A difference in reporter activity was evaluated between the pGL3-MSH3-2658-20 and the other constructs using the Student t-test. *, P<0.01; **, P<0.05. B: The effect of DPE on transcriptional activity. Reporter constructs with and without DPE sequences were compared in SW620 cells. *, P<0.01; **, P<0.05. C: Consensus sequences for TATA-Box (TATAWAAR), Int (YYANWYY), and DPE (RGWYVT) within the core promoter region of hMSH3. A nucleotide sequence from −31 to +39 of the core promoter region of hMSH3 (lower line) and the positions of each consensus sequence are shown. The transcription initiation site is expressed as 1.