Table 2.
Cell line | Origin | p53 Status | mRNA level, %a
|
Down-regulation pattern | |
---|---|---|---|---|---|
Day 1 | Day 3 | ||||
HCT116+5 | colorectal cancer | wild type | 77.2 | 33.3b * | Rapid |
HCT116+3+5 | colorectal cancer | wild type | 80.9 | 41.5* | Rapid |
HEK293 | embryonic kidney | wild type | ntc | 32.6* | Rapid |
SW480 | colorectal cancer | mutant | 76.9 | 68.8* | Moderate |
SW620 | colorectal cancer | mutant | 73.8 | 75.3b* | Moderate |
colo320DM | colorectal cancer | mutant | nt | 73.8 | Moderate |
HeLa S3 | cervix carcinoma | mutant | nt | 79.4* | Moderate |
TGW | neuroblastoma | mutant | nt | 69.5* | Moderate |
GOTO | neuroblastoma | wild type | 107.5 | 96.2 | No |
MCF-7 | breast adenocarcinoma | wild type | 103.6 | 100.6 | No |
P<0.05
Percentage of the relative mRNA amount in hypoxia was determined by the following formula: Ct at hypoxia/Ct at normoxia×100.
The mRNA level was determined at day 2 in hypoxia.
nt: not tested.