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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Cancer Prev Res (Phila). 2009 Jul 28;2(8):732–742. doi: 10.1158/1940-6207.CAPR-08-0197

Fig. 5.

Fig. 5

A, fluorescence microscopy of Caco-2 and HT-29 cells stained with JC1, a mitochondrial potential marker stain, 4 h postsupplementation with DHA and EPA. Control cells show an orange coloration, indicating polarization of the mitochondrial membrane, and DHA and EPA-treated cells show a green coloration, as a result of mitochondrial membrane depolarization. B, immunoblotting of protein cytosolic fraction with cytochrome c and Smac/DIABLO antibodies. The increase of cytochrome c and Smac/DIABLO in the cytosolic fraction reflects their translocation to the cytosol. C, immunoblotting of cytosolic and mitochondrial fractions with cytochrome c oxidase antibody, used as a mitochondrial marker, to prove actual separation of the two fractions. Left, 2 h post-EPA supplementation; middle and right, 4 and 8 h post-DHA supplementation. D, immunoblotting with DR5, FAS, and TNF-R1 antibodies, as well as control β-actin 4 and 8 h after DHA and EPA supplementation. There is an increase of expression of TNF-R1, 8 h after fatty acid supplementation in relation to control. The table represents actual fluorescence intensity, as analyzed by FACS scan, of TNF-R1, 4 and 8 h post-EPA supplementation. The results are coincidental with the immunoblots.