Figure 3. Glast-creERT and FoxD1-cre labeled cells were not appreciably co-labeled with SMA or with vW in hind limbs.
A) Glast-creERT labeled cells were closely associated with α-smooth muscle actin (SMA)-expressing cells but no co-labeling was observed. Insert shows a typical high power image of semi-longitude section of a small blood vessel. B) FoxD1-cre labeled cells also closely associate with SMA but no co-labeling was observed. Insert shows a typical high power image of semi-cross section of a small blood vessel. C) Glast-creERT labeled cells were closely associated with von Willebrand factor (vW) expressing cells but no co-labeling was observed. Insert shows a typical high power image of a small cluster of small blood vessels. D) FoxD1-cre labeled cells were closely associated with vW but no co-labeling was observed. Insert shows a typical high power image of semi-longitude section of a small blood vessel. E&F) show the corresponding histological images of A&C, and B&D, respectively. The black arrows in E&F) point to blood vessels in muscle tissue. Bar=100μm.