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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Kidney Int. 2008 Aug 13;74(10):1310–1318. doi: 10.1038/ki.2008.395

Figure 3. Addition of NGAL induces apoptosis in Pkd1 null cells.

Figure 3

(a) A total of 1×104 Pkd1 null cells were plated and allowed to grow for 48 h±NGAL (20 μg/ml) before trypsinization and counting; n=6 (3 each for PN18 and PN24), *P<0.005. (b) Cells were plated at equal density and grown for 48 h±NGAL, and labeled with BrdU for 30 min before harvest and FACS analysis; n=11 (6 for PN18 and 5 for PN24). (c) Cells were plated at equal density and serum starved for 48 h before labeling with Annexin V PE and FACS analysis; n=9 (4 for PN18 and 5 for PN24), *P<0.01. (d) Cells were treated for 24 h±NGAL and whole cell lysates immunoblotted for the phosphorylated form of Erk (pErk) and for total Erk (Erk). (e) Quantitation of activated Erk normalized to total Erk for vehicle control and NGAL-treated cells as in (d); n=4, *P<0.01.