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. 2013 Sep 22;2013:268249. doi: 10.1155/2013/268249

Figure 2.

Figure 2

Screening of S. cerevisiae transformants. (a) S. cerevisiae transformed with pB2ZB2-SK expression vector were selected on YPD-Zeocin (200 μg/mL) medium. Transformants with pB2ZB2-SK vector (Lane 1), untransformed S. cerevisiae (Lane 2). (b) Colony PCR (P3 and P4 primers) of Saccharomyces transformants selected on YPD Zeocin medium. Untransformed S. cerevisiae (Lane 1), transformants with 1.5 Kbp skc expression cassette fragments (Lanes 2, 3, 4, and 5) and 0.25–12.0 Kbp DNA ladder (Lane 6).