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. 2013 Sep 22;2013:268249. doi: 10.1155/2013/268249

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Copyright © 2013 Ravi N. Vellanki et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Southern blot analyses of S. cerevisiae-SK clone. Genomic 635 DNA of the clone was restricted with SapI/AgeI enzymes, blotted, and hybridized with α32P-labelled 1.63 Kbp P_GAP-skc-_AOXTT cassette fragment. S. cerevisiae clone genomic DNA extracted at 24 (Lane 2), 72 (Lane 3), 96 (Lane 4), and 120 hrs (Lane 5) and untransformed S. cerevisiae genomic DNA (Lane 1). 1.63 Kbp P_GAP-skc-_AOXTT cassette fragment is specified.