Fig. 3.

Thiol reduction of KYC disulfide. KYC (440 μM) was mixed with MPO (50 nM) and H₂O₂ (100 μM) for 2 h (as in Fig. 2A, trace b) and reaction products were analyzed by HPLC (trace a). An aliquot (50 μl) of the reaction mixture in trace a was mixed with 10 mM GSH (50 μl) for 120 min and the resultant products analyzed by HPLC (trace b). An aliquot (50 μl) of the reaction mixture in trace a was mixed with 50 μl of DTT (0.5 mM) for 5 min at room temperature and the products analyzed by HPLC (trace c).