Fig. 5.

The protection of BAECs by KYC from MPO-induced injury. BAECs (passages 6–8) were cultured in 24-well plates with DMEM and 10% FBS until 70–80% confluent and then treated with MPO, H₂O₂, and KYC as described in Materials and Methods. Images are representative of three independent studies. A: KYC concentration-dependent protection against MPO-induced BAEC injuries. B: BAECs incubated with 2.5 μg MPO and 100 μM H₂O₂ with or without 25 μM KYC for 30 min. C: BAECs incubated with 5 μg MPO and 50 μM H₂O₂ with or without 25 μM KYC for 30 min. D: KYC concentration-dependent protection against PMA-stimulated neutrophil-induced BAEC injury.